Characterization of Copper Bioavailability in Wheat Flour by Chemical Fractionation and Inductively Coupled Plasma-Mass Spectrometry


SEVEN ERDEMİR Ü., Gucer S.

ANALYTICAL LETTERS, vol.49, no.7, pp.1056-1065, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 7
  • Publication Date: 2016
  • Doi Number: 10.1080/00032719.2015.1065878
  • Journal Name: ANALYTICAL LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1056-1065
  • Keywords: fractionation, Bioavailability, inductively coupled plasma-mass spectrometry, wheat flour, copper, ATOMIC-ABSORPTION-SPECTROMETRY, CORYLUS-AVELLANA L., ORAL BIOACCESSIBILITY, WATER SOLUBILITY, TRACE-ELEMENTS, ICP-MS, METALS, MANGANESE, FOOD, CONSTITUENTS
  • Bursa Uludag University Affiliated: Yes

Abstract

The bioavailability of copper in wheat flour is reported based on chemical fractionation using water, diethyl ether, n-hexane, methanol, acetone, and chloroform/methanol to identify copper-bound matrix sites. The total copper concentrations in the fractions were determined by inductively coupled plasma-mass spectrometry. In vitro gastrointestinal digestion was performed using pepsin and pancreatin enzyme solutions at defined pH values for bioavailability studies. The bioavailable forms of copper in the samples were 61-80% of the total concentration. Extraction with n-octanol and activated carbon adsorption studies were evaluated to compare their efficiencies to the enzymatic approach.