Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa
CRYOBIOLOGY, vol.72, no.3, pp.269-273, 2016 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 72 Issue: 3
- Publication Date: 2016
- Doi Number: 10.1016/j.cryobiol.2016.03.007
- Journal Name: CRYOBIOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.269-273
- Keywords: Freeze-drying, Goat semen, Cryopreservation, Antioxidants, Incubation resilience, RAM SEMEN FROZEN, SOYBEAN LECITHIN, SPERM PARAMETERS, CRYOPRESERVATION, FERTILITY, INTEGRITY, ACROSOME, AGENTS
- Bursa Uludag University Affiliated: Yes
Abstract
The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine. (C) 2016 Elsevier Inc. All rights reserved.