Antioxidant Activity Assay of Phenolic Compounds Isolated from Origanum Onites L. Aromatic Water by High Performance Liquid Chromatography

Creative Commons License

Aybastıer Ö., Demir C.

Annals of Nutritional Disorders & Therapy, vol.4, no.3, pp.1-4, 2017 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 4 Issue: 3
  • Publication Date: 2017
  • Journal Name: Annals of Nutritional Disorders & Therapy
  • Journal Indexes: Other Indexes
  • Page Numbers: pp.1-4
  • Bursa Uludag University Affiliated: Yes


A High Performance Liquid Chromatographic (HPLC) method was developed to investigate the antioxidant performance of Origanum onites L. aromatic water and its phenolic compounds in terms of the free radical scavenging activity in Fenton reaction. The remaining concentrations of carvacrol, thymol and thymoquinone were measured after the oxidation reaction by HPLC to evaluate the scavenging potential of these compounds for reactive hydroxyl radicals. The main oxidation product of thymoquinone showed the highest scavenging activity in Fenton reaction. The aromatic water of Origanum onites L. could be used as a rich source of natural antioxidants with potential applications in pharmaceutical industry.