ISOLATION OF MAJOR PHENOLIC COMPOUNDS FROM THE EXTRACTS OF PRUNELLA L. SPECIES GROWN IN TURKEY AND THEIR ANTIOXIDANT AND CYTOTOXIC ACTIVITIES


ŞAHİN S. , ARI F. , Demir C., Ulukaya E.

JOURNAL OF FOOD BIOCHEMISTRY, vol.38, no.2, pp.248-257, 2014 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 2
  • Publication Date: 2014
  • Doi Number: 10.1111/jfbc.12043
  • Title of Journal : JOURNAL OF FOOD BIOCHEMISTRY
  • Page Numbers: pp.248-257

Abstract

The major phenolics of PrunellaL. species were isolated, and antioxidant activites were determined by 2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid). The isolated compounds were identified as rutin, rosmarinic acid and caffeic acid by high performance liquid chromatography-diode array detection. The results show that the phenolic compounds were isolated from PrunellaL. species with high content ranged from 74% (Prunella grandifloraL.) to 99% (Prunella orientalisBornm.), and the major compound of rosmarinic acid was isolated above 90% in all species. Antigrowth effects of PrunellaL. species were tested against human breast cancer cell lines, MCF-7 (estrogen receptor-positive) and MDAMB-231 (estrogen receptor-negative) by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) and ATP (adenosine triphosphate) viability assays. To determine the mode of cell death, M30-antigen assay was performed in order to measure apoptosis. It was found that Prunella laciniata (L.) L., P.orientalisBornm. and P.grandifloraL. species showed antigrowth effect on MCF-7 and MDA-MB-231 breast cancer cells, but apoptosis was not observed.