Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa

ALÇAY S., Gokce E., Toker M. B., Onder N. T., ÜSTÜNER B., UZABACI E., ...More

CRYOBIOLOGY, vol.72, no.3, pp.269-273, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 72 Issue: 3
  • Publication Date: 2016
  • Doi Number: 10.1016/j.cryobiol.2016.03.007
  • Journal Name: CRYOBIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.269-273
  • Keywords: Freeze-drying, Goat semen, Cryopreservation, Antioxidants, Incubation resilience, RAM SEMEN FROZEN, SOYBEAN LECITHIN, SPERM PARAMETERS, CRYOPRESERVATION, FERTILITY, INTEGRITY, ACROSOME, AGENTS
  • Bursa Uludag University Affiliated: Yes


The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine. (C) 2016 Elsevier Inc. All rights reserved.