ISOLATION OF MAJOR PHENOLIC COMPOUNDS FROM THE EXTRACTS OF PRUNELLA L. SPECIES GROWN IN TURKEY AND THEIR ANTIOXIDANT AND CYTOTOXIC ACTIVITIES

ŞAHİN S., ARI F., Demir C., Ulukaya E.

JOURNAL OF FOOD BIOCHEMISTRY, cilt.38, sa.2, ss.248-257, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 2
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1111/jfbc.12043
  • Dergi Adı: JOURNAL OF FOOD BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.248-257
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The major phenolics of PrunellaL. species were isolated, and antioxidant activites were determined by 2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid). The isolated compounds were identified as rutin, rosmarinic acid and caffeic acid by high performance liquid chromatography-diode array detection. The results show that the phenolic compounds were isolated from PrunellaL. species with high content ranged from 74% (Prunella grandifloraL.) to 99% (Prunella orientalisBornm.), and the major compound of rosmarinic acid was isolated above 90% in all species. Antigrowth effects of PrunellaL. species were tested against human breast cancer cell lines, MCF-7 (estrogen receptor-positive) and MDAMB-231 (estrogen receptor-negative) by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) and ATP (adenosine triphosphate) viability assays. To determine the mode of cell death, M30-antigen assay was performed in order to measure apoptosis. It was found that Prunella laciniata (L.) L., P.orientalisBornm. and P.grandifloraL. species showed antigrowth effect on MCF-7 and MDA-MB-231 breast cancer cells, but apoptosis was not observed.