Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Bursa Uludağ Üniversitesi, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ, Türkiye
Tezin Onay Tarihi: 2001
Tezin Dili: Türkçe
Öğrenci: FATMA ARI
Danışman: Egemen Dere
Özet:
The In Vitro Effect of Benzene on Liver Glutathione S-transferase Enzyme
Activity" In this study, the effect of benzene which is known as a
carcinogen and toxic agent on liver Glutathione S-transferase (GST) enzyme was
studied in vitro. GST enzyme activity was determined by following the formation
of l-chloro-2,4- dinitrobenzene -Glutathione (CDNB-GSH) conjugate with
spectroscopic method. Our results showed that there is an inverse correlation
between GST conjugation activity and concentration of benzene, and reaction
time. Variations in the Vmax and Km value for CDNB of the enzyme was observed.
The results obtained showed that GST activity for CDNB-GSH conjugation reaction
was inhibited with mixed reaction mechanism by benzene. Key Words : Benzene,
Glutathione, Glutathione S-transferase