Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Bursa Uludağ Üniversitesi, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ, Türkiye
Tezin Onay Tarihi: 2018
Tezin Dili: Türkçe
Öğrenci: EMİNE GONCA PEKEL
Danışman: Egemen Dere
Özet:
Post-mortem biochemical investigations contribute to the determination of post-mortem biochemical changes in the organism and to the investigation of cause of death and death time, ante-mortem and post-mortem processes. It is important to reach new information in this area with the researches to be done with different markers. In this study, toluene was used which is a common volatile compound used in the industrial field and in daily life, which is also used abused solvent. In case of people exposed to toluene, serious damage occurs in many tissues and organs. In addition, toxic effects caused by exposure at high doses can lead to death. Reactive oxygen species are produced as a result of toluene metabolism. Antioxidants work in defense against these reactive oxygen species. In this study, the effects of deaths caused by toluene exposure in rats on antioxidant enzymes glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) activities and post-mortem time dependent changes of these enzymes were investigated. For this purpose, 30 Wistar-albino male rats were divided into a control group and an experimental group. In the experimental group, rats were injected with toluene and death due to toluene exposure was performed. In the control group, saline injection was performed instead of toluene. At the end of post-mortem 0, 6, 12, 24 and 48 hours, liver tissues and heart blood samples of control and experimental groups were collected, then GSH-Px, CAT, and SOD activities were measured. As a result, GSH-Px, SOD, and CAT activities in the liver samples of experimental group and GSH-Px activity in the blood samples of experimental group were significantly higher than the control group. Depending on the post-mortem time, an increase in enzyme activities was observed in GSH-Px and CAT, especially in the 12-48 hour period. The results show that the toxic effect of acute toluene exposure at high doses and the antioxidant response. Also, the increase in enzyme activity in the post-mortem range of 12-48 hours may be due to autolysis after death.
Keywords: Post-mortem biochemistry, toluene, glutathione peroxidase, catalase, superoxide dismutase
2018, vii