Research Information System
Thesis Type: Doctorate
Institution Of The Thesis: Uludağ Üniversitesi, Turkey
Approval Date: 2018
Thesis Language: Turkish
Student: ARDA SÖZCÜ
Supervisor: İBRAHİM AK
Open Archive Collection: AVESIS Open Access Collection
Abstract:This study was carried out with the aim of determining the effect of in-ovo injection of glutamine on embryo development, hatching parameters, intestinal development, blood parameters, chick quality, broiler performance and carcass characteristics. In the study, hatching eggs were obtained from 36 weeks of age of Ross 308 broiler breeder flock. The eggs were injected with liquid form of L-glutamine into air sac on day 17th day of incubation. Six treatment groups were created as control (no injection), injection with distilled water (shame group), injection with 20 mg (dose 1), 40 mg (dose 2), 60 mg (dose 3) and 80 mg (dose 4) glutamine dissolved in 0,5 ml distilled water. Embryonic growth traits were measured on day 20th of incubation, whereas chick quality parameters, organ development, intestinal morphology, blood parameters (glucose level, immunoglobulins, liver enzymes and blood cells), survival rate and late term embryonic mortalities were determined at hatch. During growing period (1-6 weeks), weekly broiler performance was determined in the treatment groups. On days 14 and 42 of growing period, organ development, intestinal morphology and blood parameters, on day 42 slaughter and carcass parameters were detected. On day 20 of incubation, the highest mean values for embryo weight, yolk absorption, embryo body and leg length were observed in dose 2 group (P<0,01). Survival rate was found to be higher in dose 1 and dose 2 groups with values of 95,2% and 96,7%, respectively, whereas cull chick rate was lower in control, dose 1 and dose 2 groups. Chick hatching weight and relative chick weight were determined as the highest in dose 2 group (respectively 45,9 g and 72,2%, respectively). At hatch, a stimulating effect og the treatment was observed in dose 2 group for villus growth in duodenum, jejenum and ileum, and immune system development. At the end of the growing period, broilers in dose 2 group was heavier with a body weight of 3 090,3 g, whereas broilers in other groups had similar body weights (P<0,01). Feed conversion rate was better in dose 1 and dose 2 groups with values of 1,65 and 1,60, whereas worse feed efficiency in shame group and dose 4 with values of 1,81 and 1,84, respectively (P<0,01). Mortality was higher with a value of 8,9% in shame and dose 4 groups. Slaughter yield was found to be higher as 77,3% and 78,2% in dose 1 and dose 2 groups (P<0,01). In conclusion, 40 mg glutamine in-ovo injection that was applied as dose 2 caused an increament in embryonic growth, improvement for chick quality, increament in survival rate, a stimulating effect for digestive system organs, villus development and immune system, improvement of growth performance parameters and a reduction in mortality.