Genetic Variability of FABP4 c.328 G>A (rs110652478) Polmorphism and Its Association with Slaugter Weight and Carcass Traits in Aberdeen Angus and Hereford Bulls Imported into Turkey

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Ardıçlı S., Üstüner H., Arslan Ö.

Journal of Research in Veterinary Medicine, vol.40, no.1, pp.10-18, 2021 (Peer-Reviewed Journal)


The study aimed to determine the genotypic distribution of the c.328 G>A (rs110652478) polymorphism in the bovine fatty acid-binding protein

4 (FABP4) gene and to determine its effect on slaughter weight in Aberdeen Angus and Hereford bulls imported into Turkey. A total of

randomly selected 95 bulls were used in this study. Bulls were housed for fattening in semi-open pens for approximately nine months with the

same management procedures. Genotyping of the FABP4 c.328 G>A (rs110652478) polymorphism, located in exon 3, was performed by the

PCR-RFLP method. Allele and genotype frequencies were calculated by the standard procedures and the Hardy-Weinberg equilibrium was tested

by comparing expected and observed genotype frequencies using chi-square and Fisher’s exact tests. Indices of genetic diversity were calculated

based on the allelic frequencies. The least-squares method as applied in a GLM procedure was used to test the association between FABP4 c.328

G>A and phenotypic traits including slaughter weight, hot and cold carcass weight, chilling loss, dressing percentage, and carcass pH. Results

revealed that the most frequent genotype was GG (49.18% and 52.94% for Angus and Herefords, respectively) and the minor allele frequency (A

allele) was 0.32 in the total population. The genotype distribution was in agreement with Hardy Weinberg equilibrium (P>0.05). The moderately

low levels of genetic variability observed in this study may be caused as a result of eventual inbreeding. PIC levels suggest that FABP4 c.328 G>A

can be a mildly informative genetic marker for the tested population. Association analysis indicated that the effect of the FABP4 on slaughter

weight was statistically significant (P<0.05) in the total population. However, this association was not substantiated in breed-specific analyzes.

There was no significant effect of the marker on any remaining phenotypic traits. Consequently, it is important to emphasize that analysis of

FABP4 c.328 G>A may provide valuable, but limited, information on imported beef cattle raised in Turkey conditions.