Biohydrogen production via integrated sequential fermentation using magnetite nanoparticles treated crude enzyme to hydrolyze sugarcane bagasse


Srivastava N., Alhazmi A., Mohammad A., Haque S., Srivastava M., Pal D. B. , ...More

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol.47, no.72, pp.30861-30871, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 72
  • Publication Date: 2022
  • Doi Number: 10.1016/j.ijhydene.2021.08.198
  • Journal Name: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Artic & Antarctic Regions, Chemical Abstracts Core, Communication Abstracts, Environment Index, INSPEC
  • Page Numbers: pp.30861-30871
  • Keywords: Iron oxide nanoparticles, Green synthesis, Reducing sugars, Dark-fermentation, Photo-fermentation, Biohydrogen, HYDROGEN-PRODUCTION, DARK FERMENTATION, REDUCING SUGARS, RICE STRAW, PHOTO, WASTE, IMMOBILIZATION, CELLULASE, WATER, PHOTOFERMENTATION
  • Bursa Uludag University Affiliated: No

Abstract

This study presents a potential approach to enhance integrated sequential biohydrogen production from waste biomass using magnetite nanoparticle (Fe3O4 NPs) which is & nbsp;synthesized through waste seeds of Syzygium cumini. Consequences of 0.5% Fe3O4 NPs have been investigated on the thermal and pH stability of fungal crude cellulase. It is noticed that Fe3O4 NPs treated enzyme and control exhibits 100% activity in the temperature range of 45-60 degrees C and 45-55 degrees C, respectively. Moreover, Fe3O4 NPs treated enzyme showed extended thermal stability in the temperature range of 50-60 degrees C up to 12 h. Beside this, Fe3O4 NPs treated enzyme possesses 100% stability in the pH range of 5.0-7.0 whereas control exhibited only at pH 6.0. Enzymatic hydrolysis via Fe3O4 NPs treated enzyme has been employed which produces-68.0 g/L reducing sugars from sugarcane bagasse. Sub-sequently, sugar hydrolyzate has been utilized as substrate in the sequential integrated fermentation that produces-3427.0 mL/L cumulative hydrogen after 408 h. This approach may have potential for the pilot scale production of biohydrogen from waste biomass at low-cost in an eco-friendly manner. (c) 2021 Published by Elsevier Ltd on behalf of Hydrogen Energy Publications LLC.