Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin-based extenders


Alçay S. , Üstüner B. , Aktar A., Mulkpinar E., Duman M. , Akkasoglu M., ...More

ANDROLOGIA, vol.52, no.4, 2020 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 52 Issue: 4
  • Publication Date: 2020
  • Doi Number: 10.1111/and.13555
  • Title of Journal : ANDROLOGIA
  • Keywords: post-thaw incubation, saanen goat, seminal plasma, sperm freezing, SOYBEAN LECITHIN, RAM SEMEN, EGG-YOLK, INCUBATION RESILIENCE, POSTTHAW QUALITY, LIQUID STORAGE, SPERMATOZOA, ANTIOXIDANT, PARAMETERS

Abstract

The objective of this study was to determine the optimum concentrations of rainbow trout seminal plasma (RTS) supplemented extenders for goat semen quality at post-thaw and after incubation. Five sexually mature Saanen goat (Capra aegagrus hircus) were used for semen collection. Pooled semen was diluted with soybean lecithin-based extender without RTS (control) or supplemented with different concentrations of RTS (1%, 2%, 4% or 8%), at a final concentration of 150 x 10(6) spermatozoon/ml. Sperm motility, plasma membrane functional integrity (HOST), damaged acrosome (PSA-FITC), mitochondrial activity (rhodamine123) and DNA integrity (TUNEL) were evaluated. Spermatological parameters were evaluated at post-thaw and after 6 hr incubation. RTS8 group preserved sperm motility, acrosomal integrity, plasma membrane functional integrity and mitochondrial function better than the control group (p < .05). The study demonstrated that RTS supplemented lecithin-based extenders have useful effects on goat spermatozoa. In addition, the results of the current study represented the positive effect of using 8% RTS supplemented extender.