Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin-based extenders

Alçay S., Üstüner B., Aktar A., Mulkpinar E., Duman M., Akkasoglu M., ...Daha Fazla

ANDROLOGIA, cilt.52, sa.4, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 52 Sayı: 4
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1111/and.13555
  • Dergi Adı: ANDROLOGIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, Gender Studies Database, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: post-thaw incubation, saanen goat, seminal plasma, sperm freezing, SOYBEAN LECITHIN, RAM SEMEN, EGG-YOLK, INCUBATION RESILIENCE, POSTTHAW QUALITY, LIQUID STORAGE, SPERMATOZOA, ANTIOXIDANT, PARAMETERS
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The objective of this study was to determine the optimum concentrations of rainbow trout seminal plasma (RTS) supplemented extenders for goat semen quality at post-thaw and after incubation. Five sexually mature Saanen goat (Capra aegagrus hircus) were used for semen collection. Pooled semen was diluted with soybean lecithin-based extender without RTS (control) or supplemented with different concentrations of RTS (1%, 2%, 4% or 8%), at a final concentration of 150 x 10(6) spermatozoon/ml. Sperm motility, plasma membrane functional integrity (HOST), damaged acrosome (PSA-FITC), mitochondrial activity (rhodamine123) and DNA integrity (TUNEL) were evaluated. Spermatological parameters were evaluated at post-thaw and after 6 hr incubation. RTS8 group preserved sperm motility, acrosomal integrity, plasma membrane functional integrity and mitochondrial function better than the control group (p < .05). The study demonstrated that RTS supplemented lecithin-based extenders have useful effects on goat spermatozoa. In addition, the results of the current study represented the positive effect of using 8% RTS supplemented extender.