Affinity binding of proteins to the modified bacterial cellulose nanofibers


Bakhshpour M., Tamahkar E., ANDAÇ A. M. , DENİZLİ A.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, vol.1052, pp.121-127, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1052
  • Publication Date: 2017
  • Doi Number: 10.1016/j.jchromb.2017.03.021
  • Journal Name: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.121-127
  • Keywords: Protein recognition, Metal ion coordination, Bacterial cellulose nanofibers, METAL-CHELATE AFFINITY, CYTOCHROME-C PURIFICATION, HUMAN BLOOD, ADSORPTION, MEMBRANE, CHROMATOGRAPHY, CRYOGELS, RECOGNITION, HEMOGLOBIN, SEPARATION
  • Bursa Uludag University Affiliated: No

Abstract

The potential of the modified bacterial cellulose (BC) nanofibers was determined bearing metal ion coordination interactions to enhance the protein adsorption and binding capacity. Thus, a household synthesized metal chelating monomer, namely N-methacryloyl-L-histidine methylester (MAH), and a commercial metal chelating monomer, namely 4-vinylimidazole (VIm), were used to complex with metal ions Cu(II) and Ni(II) respectively for the synthesis of the modified BC nanofibers. The modified nanofibers were characterized by FT-IR, SEM and EDX measurements. The protein adsorption tests were carried out using hemoglobin as a model protein and it was determined that the maximum adsorption capacity of hemoglobin onto the modified BC nanofibers was found as 47.40 mg/g. The novel strategy for the preparation of metal chelated nanofibers was developed. (C) 2017 Elsevier B.V. All rights reserved.