Aim One-Lung Ventilation (OLV) is widely used in a variety of cardiac, thoracic, and major vascular procedures to ventilate an isolated lung field in humans and dogs. Endobronchial tubes, endobronchial blockers, and single-lumen tubes are commonly using equipments for OLV. Material and Methods Conventional one-lung intermittent positive-pressure ventilation (OL-IPPV) has been a valuable technique during anaesthesia for intrathoracic operations in humans and dogs. Here, our experience resulted in the usage of Pezzer catheter to establish OLV in dogs, while performing tracheobronchial resection. OLV was successfully established with Pezzer catheter in 12 dogs. All application was performed by the same surgeon. An optimal diameter Pezzer catheter was selected to insert into the lumen of the trachea and the main bronchus. The length of the catheter was prepared and middle point of the head of the catheter was cut at the level of holes on it. Following tracheobronchial resection, the cut head was inserted into the trachea with a clamp and end of the catheter was instantly inserted the main bronchus (Figure 1). Sutures were prepared interruptedly and passed between the trachea and the main bronchus. Before sutures were started the ligation tightly, Pezzer catheter was removed in the lumen and than ligations were completed. Results No accidental episode was encountered during the manipulation. OLV was successfully established, and the mean oxygen saturation range and end tidal carbon dioxide concentration was 95% and 37-41% before and after insertion of the Pezzer catheter, respectively. The respiratory modes during the surgery were not need to change. Discussion OLV is essential in the tracheaobronchial resections. Here, the Pezzer catheter is presented to obtain OLV. It has been concluded that Pezzer catheter is a very practical and reliable method and it can be choice for cases which require OLV.