The Effect of Capsaicin on IGF-I and IGF-IR Expression in Ovarian Granulosa Cells


Guler S. , Zik B.

JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY, vol.71, no.1, pp.1977-1984, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 71 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.12681/jhvms.22939
  • Title of Journal : JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY
  • Page Numbers: pp.1977-1984
  • Keywords: ovary, capsaicin, IGF-I, IGF-IR, GROWTH-FACTOR-I, FOLLICLE-STIMULATING-HORMONE, GENE-EXPRESSION, INSULIN, RECEPTOR, STEROIDOGENESIS, PROLIFERATION, TRPV1, METHOXYCHLOR, PEPPER

Abstract

Capsaicin (trans-8-methyl-N-vanillyl-6-noneadamide) is a pungent ingredient in red peppers from the Capsicum family. Insulin-like growth factor-I (IGF-I) is expressed in granulosa cells and has an important role in ovarian development. However, there are no data about the IGF-I expression in ovarian granulosa cells after capsaicin treatment. The aim of this study was to investigate the expression of IGF-I and its receptor (insulin-like growth factor-I receptor [IGF-IR]) in primary rat ovarian granulosa cells after low and high doses of capsaicin treatment. For this, granulosa cells were isolated and cultured from ovaries of 30-day-old female Sprague-Dawley rats. Granulosa cell plates were divided into four groups as cell control (C), vehicle control (V), and 50 mu M and 150 mu M capsaicin groups. In experimental groups, granulosa cells were exposed to capsaicin for 24 hours and immunocytochemistry was performed afterwards using anti-IGF-I and anti-IGF-IR antibodies. Both IGF-I and IGF-IR expressions were found to be significantly increased in parallel to the capsaicin doses. Elevated levels of IGF-I may be a risk factor for ovarian development. Because of the crucial role of IGF-I in ovary development, capsaicin treatment can be effective on follicular development and/or disorders characterized by high IGF-I levels.