Antimicrobial resistance and resistance genes in Flavobacterium psychrophilum isolates from Turkey

Satıcıoğlu İ. B., Duman M., Smith P., Wiklund T., Altun S.

AQUACULTURE, vol.512, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 512
  • Publication Date: 2019
  • Doi Number: 10.1016/j.aquaculture.2019.734293
  • Journal Name: AQUACULTURE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Antimicrobial resistance genes, Flavobacterium psychrophilum, floR, NRI, sul2, TETRACYCLINE RESISTANCE, IN-VITRO, SUSCEPTIBILITY, BACTERIA, VALIDATION, MUTATIONS, PRIMERS, DISEASE, WATER
  • Bursa Uludag University Affiliated: Yes

Abstract

The antimicrobial susceptibilities of Turkish F. psychrophilum isolates were investigated using a Clinical and Laboratory Standards Institute (CLSI) standard microdilution testing protocol. The significance of the minimum inhibitory concentrations was determined by applying empirically and statistically determined epidemiological cut-off values calculated by normalized resistance analysis. Twenty-five F. psychrophilum isolates made over four years from three different trout species in five different regions were examined. All isolates were fully susceptible to amoxicillin and florfenicol, but 88% of our isolates were categorized as having reduced susceptibility to the two quinolones, oxolinic acid, and enrofloxacin. Eighty percent of the isolates were categorized as showing reduced susceptibility to oxytetracycline.