In vitro Antigenotoxicity of Ulva rigida C. Agardh (Chlorophyceae) Extract against Induction of Chromosome Aberration, Sister Chromatid Exchange and Micronuclei by Mutagenic Agent MMC

ÇELİKLER KASIMOĞULLARI S., YILDIZ G., VATAN Ö., Bilaloglu R.

BIOMEDICAL AND ENVIRONMENTAL SCIENCES, cilt.21, sa.6, ss.492-498, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 6
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/s0895-3988(09)60008-8
  • Dergi Adı: BIOMEDICAL AND ENVIRONMENTAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.492-498
  • Anahtar Kelimeler: Ulva rigida, Anticlastogenicity, Antigenotoxicity, Chromosomal aberration, Sister chromatid exchange, Micronuclei, Mitomycin-C, WAKAME UNDARIA-PINNATIFIDA, ANTIBACTERIAL ACTIVITY, MARINE-ALGAE, NATURAL-PRODUCTS, SEAWEED, CELLS, PURIFICATION, ANTIOXIDANTS, CAROTENOIDS, CARCINOMA
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Objective To determine the in vitro possible clastogenic and cytotoxic activities of Ova rigida crude extracts (URE), and identify their antigenotoxic and protective effects on chemotherapeutic agent mitomycine-C (MMC). Methods Anti-clastogenic and anti-genotoxic activities of Ulva rigida crude extracts (URE) were studied using chromosome aberration (CA), sister chromatid exchange (SCE), and micronuclei (MN) tests in human lymphocytes cultured in vitro. Results The chromosome aberration, sister chromatid exchange or micronuclei tests showed that URE at concentrations of 10, 20, and 40 mu g/mL had no clastogenic activity in human lymphocyte cell Culture. Three doses of URE significantly decreased the number of chromosomal aberrations and the frequencies of SCE and MN when compared with the culture treated with MMC (P<0.0001). Conclusion Although URE itself is not a clastogenic or cytotoxic substance, it possesses strong antigenotoxic, anti-clastogenic, and protective effects on MMC in vitro.