Comparing the effects of treatment with ammonium molybdate versus ammonium molybdate and phenoxy-2-methyl-2-propionic acid on liver functions in natural copper poisoning of sheep


MECİTOĞLU Z., TOPAL O., KAÇAR Y., BATMAZ H.

SMALL RUMINANT RESEARCH, vol.150, pp.93-96, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 150
  • Publication Date: 2017
  • Doi Number: 10.1016/j.smallrumres.2017.03.010
  • Journal Name: SMALL RUMINANT RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.93-96
  • Keywords: Copper poisoning, Sheep, Ammonium molybdate, Phenoxy-2-methyl-2-propionic acid, (PMPA), AST, GGT, PEROXISOME PROLIFERATOR NAFENOPIN, ACTIVATED RECEPTOR-ALPHA, LIPID-PEROXIDATION, PROPIONIC-ACID, FATTY LIVER, DAIRY-COWS, METABOLISM, TOXICOSIS, TETRATHIOMOLYBDATE, MOLYBDENUM
  • Bursa Uludag University Affiliated: Yes

Abstract

Aim of the presented study is to compare the effects of treatment with ammonium molybdate versus ammonium molybdate and phenoxy-2-methyl-2-propionic acid on liver functions in natural copper poisoning of sheep and overall treatment responses in sheep naturally poisoned with copper (Cu). Study was conducted on 80 yearlings aging between 6-9 months. AM + PMPA group (n =50) received ammonium molybdate and PMPA and AM group (n =30) received only ammonium molybdate. First blood samples were collected before the treatments. PMPA was administered once daily intramuscularly at dose of 10 mg/kg for the first three days of the study to AM + PMPA group. AM + PMPA and AM groups both received ammonium molybdate two times with one week interval at dose of 1.34 mg/kg (1 cc per 10 kg BW, of %1.34 ammonium molybdate in saline solution) subcutaneously. Second blood samples were collected from all 80 animals on day 21 of the study. Cu levels were measured in a subgroup of randomly selected 9 (5 from AM + PMPA and 4 from AM group) animals on days 0 and 21 of the study. Mean Cu levels were 158.25 +/- 14 mu g/dl and 156.75 +/- 9 mu g/dlon day 0 and 129 +/- 9 mu g/dl and 154.5 +/- 22 mu g/dl in AM + PMPA and AM group respectively. AST levels decreased from 502 +/- 67.2114 to 168 +/- 10.1 IU/L in AM + PMPA group (P < 0.001) and from 423 +/- 71.1 IU/Lto 202 +/- 17.1 IU/L in AM group (P = 0.005) on day 21 of the study. GGT levels were 250 +/- 24.2 IU/L and 248 +/- 28.1 IU/L on day 0 and decreased to 160 +/- 16.41 U/L and 166 +/- 22.2 IU/L on day 21 in AM + PMPA and AM group with significance of P=0.001 and P=0.037 respectively. Two animals from AM group and one from AM + PMPA group died during the study period. Based on the more pronounced decrease in AST and GGT levels in AM + PMPA group we conclude that PMPA has beneficial effects on liver functions in chronic copper poisoning of sheep probably as a result of decreased lipid peroxidation in hepatocytes and/or increased Cu elimination by cholerectic effects of PMPA. (C) 2017 Elsevier B.V. All rights reserved.