Effects of citicoline used alone and in combination with mild hypothermia on apoptosis induced by focal cerebral ischemia in rats.

Sahin S., Alkan T., Temel Ş. G., Tureyen K., Tolunay S., Korfali E.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, cilt.17, sa.2, ss.227-31, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.jocn.2009.05.016
  • Dergi Adı: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.227-31
  • Anahtar Kelimeler: Apoptosis, Cerebral ischemia, Citicoline, Hypothermia, Rat, MODERATE THERAPEUTIC HYPOTHERMIA, TRANSIENT FOREBRAIN ISCHEMIA, CDP-CHOLINE, ARTERY OCCLUSION, CASPASE ACTIVATION, CELL-DEATH, BRAIN, REPERFUSION, METABOLISM, PATHWAY
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The effects of citicoline used either alone or in combination with hypothermia on the suppression of apoptotic processes after transient focal cerebral ischemia were investigated. Middle cerebral artery occlusion (MCAo) was performed for 2 hours on Sprague-Dawley (SD) rats using intraluminal thread insertion. The treatment groups were as follows: Group 1, sham-operate 1; Group 2, saline; Group 3, citicoline (400 mg/kg intraperitoneal.); Group 4, hypothermia (34 +/- 1 degrees C); Group 5, citicoline + hypothermia. All rats were reperfused for 24 hours, and after sacrifice and transcardiac perfusion, immunohistochemical (mean standard deviation, 0.71 +/- 0.75) was lower compared to Groups 3, 4 and 5 (2.33 +/- 0.81; 3.00 +/- 0.00; 2.20 +/- 0.83; p < 0.05). There was higher expression of cispase-3 proteins in Group 2 (2.28 +/- 0.95) compared to Group 5 (1.50 +/- 0.83; p < 0.05). Bax proteins were also increased in Group 2 (1.85 +/- 1.06) compared to Group 5 (0.40 +/- 0.54) and in Group 4 (2.00 +/- 0.00) compared to Group 5 (0.40 +/- 0.54; p < 0.05). Significant differences in caspase-9 immunostaining scores were found in Group 2 (2.29 +/- 0.96) compared to Group 5 (0.20 +/- 0.44) (p < 0.05); Group 3 (1.00 +/- 0.70) compared to Group 5 (0.20 +/- 0.44: p, < 0.05); and Group 4 (3.00 +/- 0.00; p < 0.05) compared to Group 5 (0.40 +/- 0.54; p < 0.05). Thus by suppressing apoptotic processes citicoline with hypothermia is more effective than ;either used alone in ameliorating cerebral damage after transient focal ischemia. (C) 2009 Elsevier Ltd. All rights reserved.