EDUVET International Veterinary Sciences Congress, Samsun, Turkey, 25 - 27 June 2021, vol.1, pp.208-209
The follicle-stimulating hormone (FSH) acts on the Sertoli cells in the seminiferous tubules of the testis and regulates spermatogenesis up to the secondary spermatocyte stage. Polymorphism of the FSH beta-subunit gene (FSHB) gene in exon 3 was significantly associated with the fresh and frozen semen quality. There is no information on this gene in Turkish native cattle breeds. Therefore, this study aimed to investigate the genotypic distribution and population genetic parameters of the single nucleotide polymorphism (SNP) at the FSHB gene in East Anatolian Red (EAR), East Anatolian Red×Holstein (EAR×H), and Zavot (Z) bulls. A total of 68 cattle including EAR (n=34), EAR×H (n=20), and Z (n=14) bulls were used. Genomic DNA was isolated from blood samples using the phenol/chloroform method. The genotyping of the SNP was carried out by the PCR-RFLP. Deviation from Hardy–Weinberg equilibrium (HWE) was calculated by using the chi-square goodness-of-fit test. Population genetics evaluation was performed for the effective allele numbers, the polymorphism information content, theoretical heterozygosity, the fixation index, and the level of possible variability realization. Moreover, biodiversity indices including the Shannon-Weaver diversity index, Simpson Dominance, Gini-Simpson Equitability, and the True Diversity were estimated. In the present study, the AA and the AB genotypes were predominant in EAR and EAR×H bulls, respectively. Zavot breed was found to be monomorphic. There was a deviation from HWE, concerning the total cattle population. The population genetics evaluation showed that the marker was moderately informative for EAR and the crossbreeds, as well as the total population. The estimation of biodiversity indices revealed an admissible diversity for the studied locus. In conclusion, the polymorphism within exon 3 of the bovine FSHB can be interpreted as a genetic marker with reliable variability in EAR and the crossbreeds, but not in Zavot cattle.