Nesfatin-1 has a role in appetite control and energy balance. The activity of the cholinergic system also is able to affect feeding behavior. Moreover, the central cholinergic system interacts with central nesfatinergic systems. The main goal of the study was to determine the effect of intracerebroventricular (icv) and intravenous (iv) administrated CDP-choline (0.5 ve 1 mu mol; icv ve 250 mg / kg; iv) on levels of plasma nesfatin-1 in the homogeneous number of male and female fasted and the satiated Wistar albino rats. The polyethylene cannula was inserted into the carotid artery and jugular vein of the rats anesthetized with sevoflurane (2-4%/100% O-2) to collect blood samples and to make iv injection, respectively. For icy treatment, the lateral ventricle of rats was cannulated with guide cannula. The basal levels of plasma nesfatin- I in the satiated rats were higher than those observed in the fasted animals. While 0.5 and 1 mu mol dose of icy and/or 250 mg/kg dose of iv injected CDP-choline increased the level of plasma nesfatin-1 in the satiated rats, plasma nesfatin-1 level of the fasted animals decreased after the same dose and route of CDP-choline injection. The current findings show that CDP-choline can influence the level of plasma nesfatin-1 in the rats. The effect of the drug was different according to the food intake of the rats. These data might suggest a potential role in CDP-choline on plasma nesfatin-1 concentration.