Aim:This study aimed to investigate the vasoactive effects of Rosa damascena Miller essential oil and its major components, geraniol and beta-citronellol, on the rat thoracic aorta. Methods:Isolated tissue bath model and Wistar rats were used to perform the experiments. Two-fold increasing concentrations (20-160 mu g/mL) of rose oil were administered to determine its vasoactive effects. Submaximal contractions were induced by PE or KCl in both endothelium-intact and -denuded segments. Time-matched control groups were also formed. To evaluate the role of geraniol and beta-citronellol, concentrations in the range of 0.4-3.2 mu g/mL and 0.8-6.4 mu g/mL were applied respectively. The statistical significance level was considered as p < 0.05. Results:All doses of rose oil applied led to vasorelaxation in thoracic aortas precontracted with PE. In precontracted thoracic aortas with KCl, the significant effect of rose oil persisted, albeit slightly diminished. When the endothelium was removed, the relaxant effect of rose oil was partially reduced, but still significant. Besides, although geraniol relaxed aortic segments at all concentrations (0.4 to 3.2 mu g/mL), beta-citronellol caused vasorelaxation at doses of 1.6, 3.2, and 6.4 mu g/mL. Conclusion:In conclusion, the first findings were obtained that rose oil can cause a vasorelaxant effect in a concentration-dependent manner in rat thoracic aorta. This effect substantially persisted in vascular segments without endothelium or precontracted with KCl. It was further shown for the first time that geraniol and beta-citronellol exert vasodilatory effects on the rat thoracic aorta. These results suggest that rose oil exhibits its vasorelaxant effect through geraniol and beta-citronellol.