The aim of the study was to examine the pre-service teachers' perceptions about Web 2.0 technologies in their learning process and to understand their acceptance levels and attitudes towards these tools. The data gathered from a sample of 101 first year pre-service science and computer teachers in the 2009 Fall semester. The survey was made up of three components: demographic data, Web 2.0 attitude scale, and Unified Theory of Acceptance and Use of Technology (UTAUT) scale. The results indicated that participants' perceptions about Web 2.0 technologies were positive and also acceptance and willingness to use these technologies was high. The learners mostly preferred to use audio/video conferencing technologies rather than the other technologies since they are more familiar with it. (C) 2010 Elsevier Ltd. All rights reserved.