Immunomodulation of human PBMCs by peptide amphiphiles
The Annual Meeting of the Swiss Society of Allergy and Immunology, Zürich, İsviçre, 27 - 28 Ağustos 2026, ss.30, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Zürich
- Basıldığı Ülke: İsviçre
- Sayfa Sayıları: ss.30
- Bursa Uludağ Üniversitesi Adresli: Evet
Özet
Peptide amphiphiles (PAs) are supramolecular biomaterials that self-assemble into nanofibrous networks resembling natural matrix components and can modulate the immune microenvironment during tissue repair. We investigated the immunomodulatory properties of a glutamic acid-based PA (PA-E3) and 2 variants, TGF-β1-binding PA-T3 and BMP-2-binding PA-B3. Hydrogels were exposed to peripheral blood mononuclear cells (PBMCs). After 48 hours, PBMC viability and spontaneous proliferation were evaluated together with anti-CD3 and anti-CD28 mAb-stimulated proliferation of CD4+ T cells, CD8+ T cells, and CD19+ B cells. Mechanistic insights were obtained by RNA-seq. Both functionalized PAs were biocompatible, with viability and spontaneous proliferation statistically indistinguishable from PA-E3. PA-E3, PA-T3, and PA-B3 induced comparable suppression in stimulated PBMCs, indicating that this reflects a shared property of the scaffold and that PA-T3 and PA-B3 do not confer additional immunosuppressive activity. Transcriptomic profiling showed that PA-B3 induced minimal transcriptional alterations, with 47 differentially expressed genes, whereas PA-T3 elicited a broader response involving 366 genes. Pathway enrichment indicated that PA-T3 modulates chemotaxis, leukocyte migration, angiogenesis, inflammatory response regulation, fibrinolysis, extrinsic apoptotic signaling, cell-cell adhesion, leukocyte proliferation, and ossification. These findings highlight the selective immune-regulatory potential of PA-T3 and support epitope-specific PAs as immune-instructive scaffolds for bone repair.