The objective of this study was to evaluate an effect of the retained placenta on reproductive performance of 222 multiparous cows. The animals with retained placenta (RP) were divided into three treatment groups (TI; n=65, TII; n=47, TIII; n=30) and control group (n=80, without RP). In RP groups, 48-72 h after calving fetal membranes were manually separated. No administered in any drugs cows in TI group. All cows in T2 and T3 groups received intrauterine 2 g chlortetracycline boluses for 3 d. All cows in T3 group received systemic antibiotic treatment consisted of 10mg/ kg oxtetracycline that was administered for 4 d and cows with fewer on day 4(th) received oxtetracycline for an additional 3 d. Reproductive performance data were collected until pregnancy diagnosis or at six months post-partum and documented were days of the first service, first service conception rate, days open, services per conception rate. No significantly differences were found in all groups for non-pregnant cows after 180 d after post-partum. All the reproductive parameters were obtained in treatment groups were significant differences than the control group (P<0.05). This study indicated that RP in multiparous cows resulted negative impact on the reproductive performance in all treatment groups.