Vincristine modulates the expression of Ki67 and apoptosis in naturally occurring canine transmissible venereal tumor (TVT)

ÖZALP R. G., Zik B., Bastan A., Peker S., Ozdemir-Salci E. S., Bastan I., ...Daha Fazla

BIOTECHNIC & HISTOCHEMISTRY, cilt.87, sa.5, ss.325-330, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 87 Sayı: 5
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3109/10520295.2012.655311
  • Dergi Adı: BIOTECHNIC & HISTOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.325-330
  • Anahtar Kelimeler: apoptosis, canine, TVT, vincristine sulfate, GROWTH, REGRESSION, SARCOMA, CHEMOTHERAPY, PROGRESSION, EFFICACY, THERAPY, DOGS, CELL
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

We investigated eight adult dogs that were brought to veterinary clinics with a history of transmissible venereal tumors (TVT). Our goal was to demonstrate the occurrence of apoptosis and the cessation of cell proliferation at every phase of scheduled chemotherapy for naturally occurring TVT. Tissue samples were collected immediately after weekly treatments with vincristine sulfate and processed for histological purposes. Sections 5 mu m thick were stained by the TUNEL reaction for apoptosis and immunostained for Ki67 as a proliferation marker. We observed that after vincristine applications, tumor cell proliferation ceased and apoptosis increased. Ki67 HSCORE values were significantly lowered after the first and second treatments with the chemotherapeutic agent compared to controls, whereas TUNEL HSCORE values were significantly higher after two applications of vincristine compared to controls. Our results suggest that scheduled vincristine sulfate applications stabilize the induction of tumor regression by inducing apoptosis and preventing cell proliferation.