Investigation of the mechanisms involved in the central effects of glucagon-like peptide-1 on ethanol-induced gastric mucosal lesions

Isbil-Buyukcoskun N., Gulec G.

REGULATORY PEPTIDES, vol.128, no.1, pp.57-62, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 128 Issue: 1
  • Publication Date: 2005
  • Doi Number: 10.1016/j.regpep.2004.12.019
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.57-62
  • Keywords: intracerebroventricular, gastroprotection, cholinergic system, nitric oxide, somatostatin, rat, ENDOGENOUS NITRIC-OXIDE, EXPERIMENTAL-MODELS, ACID-SECRETION, GLP-1, RELEASE, SOMATOSTATIN, AMYLIN, BRAIN, GENE, ADRENOMEDULLIN
  • Bursa Uludag University Affiliated: Yes


The aim of this study was to investigate the effects of intracerebroventricularly injected glucagon-like peptide-1 (GLP-1) on ethanol-induced gastric mucosal damage and to elucidate the mechanisms involved. Absolute ethanol was administered through an orogastric cannula 5 min before GLP-1 (1 mu g/10 mu l) injection. One hour later, the rats were decapitated, their stomachs were removed and scored for mucosal damage. GLP-1 inhibited the ethanol-induced gastric mucosal damage by 92%. Centrally injected atropine sulphate, a muscarinic receptor antagonist (5 mu g/10 mu l), prevented the gastroprotective effect of GLP-1, while mecamylamine, a nicotinic receptor antagonist (25 mu g/10 mu l), was ineffective. Peripherally injected atropine methyl nitrate (1 mg/kg) did not change the effect of GLP-1, but mecamylamine (5 mg/kg) blocked it. Cysteamine, a somatostatin depletor (280 mg/kg, s.c.), did not affect the protective activity of GLP-1, while inhibition of nitric oxide (NO) synthesis by L-NAME (3 mg/kg, i.v.) significantly abolished the protective effect of GLP-1 on ethanol-induced gastric mucosal lesions. We conclude that central muscarine and peripheral nicotinic cholinergic receptors and NO, but not somatostatin, contribute to the protective effect of intracerebroventricularly injected GLP-1 on ethanol-induced gastric mucosal damage. (c) 2004 Elsevier B.V. All rights reserved.