Akademik Veri Yönetim Sistemi
The Festival of Genomics & Biodata, London, Birleşik Krallık, 26 - 29 Ocak 2021
MicroRNAs
(miRNAs) are about 22 nucleotides long and small non-coding RNAs, which take
part in multiple cellular processes by regulating gene expressions.
Dysregulated expression of miRNAs contributes to the pathogenesis of a variety
of human diseases. Possible dietary intake of plant and animal food-derived
miRNAs (dietary miRNAs) into the human circulation has been extensively
explored and debated. MiRNAs are expressed abundantly in human, bovine,
porcine, and goat milk, and a significant number of milk-derived miRNAs are
known to involve in different disease pathways. Bovine milk contains many
components, including cytokines, chemokines, hormones, growth factors, nucleotides, peptides,
enzymes. Milk-derived miRNAs are highly conserved across species and can be
transferred to humans and modulate gene expression. Our aim in this study is to
produce disease-preventive milk products modified through genetic engineering
strategies. Mesenchymal stem cells will be targeted for this RNA interference
(RNAi) transfer. Cells are isolated from tissue or circulation based on their
surface antigens (CD24, CD29, CD49f, CD10) with fluorescence-activated cell
sorting (FACS). Oncogenic miRNAs (oncomiRs) that are homolog to human miRNAs
will be knocked out in the bovine genome via RNAi in cell culture. Modified stem
cells that are successfully transferred in vitro will be injected into
the lactating bovine. The suggested miRNA-modified
milk method may provide a novel approach for
translational applications in cancer management. If our
project is completed successfully, personalized-dietary interventions can be
designed not only for cancer but also for many diseases. It offers valuable
insight into the implications of animal-derived miRNAs on human health.