The Festival of Genomics & Biodata, London, United Kingdom, 26 - 29 January 2021
MicroRNAs (miRNAs) are about 22 nucleotides long and small non-coding RNAs, which take part in multiple cellular processes by regulating gene expressions. Dysregulated expression of miRNAs contributes to the pathogenesis of a variety of human diseases. Possible dietary intake of plant and animal food-derived miRNAs (dietary miRNAs) into the human circulation has been extensively explored and debated. MiRNAs are expressed abundantly in human, bovine, porcine, and goat milk, and a significant number of milk-derived miRNAs are known to involve in different disease pathways. Bovine milk contains many components, including cytokines, chemokines, hormones, growth factors, nucleotides, peptides, enzymes. Milk-derived miRNAs are highly conserved across species and can be transferred to humans and modulate gene expression. Our aim in this study is to produce disease-preventive milk products modified through genetic engineering strategies. Mesenchymal stem cells will be targeted for this RNA interference (RNAi) transfer. Cells are isolated from tissue or circulation based on their surface antigens (CD24, CD29, CD49f, CD10) with fluorescence-activated cell sorting (FACS). Oncogenic miRNAs (oncomiRs) that are homolog to human miRNAs will be knocked out in the bovine genome via RNAi in cell culture. Modified stem cells that are successfully transferred in vitro will be injected into the lactating bovine. The suggested miRNA-modified milk method may provide a novel approach for translational applications in cancer management. If our project is completed successfully, personalized-dietary interventions can be designed not only for cancer but also for many diseases. It offers valuable insight into the implications of animal-derived miRNAs on human health.