Akademik Veri Yönetim Sistemi
Acta Pharmaceutica, cilt.51, sa.3, ss.201-209, 2001 (SCI-Expanded)
The present paper describes methods for direct determination of manganese in human serum and urine by electrothermal atomic absorption spectrometry (etaas) with both deuterium and Zeeman background corrections and with nitric acid as matrix modifier. In both deuterium and Zeeman correction methods, the presence of nitric acid significantly reduces the background signal and direct determination of manganese is possible. Pyrolysis temperature of 900 °c was found optimal for deuterium and 1000 °c for Zeeman correction, while 1800 °c and 2100 °c were found to be optimal atomizing temperatures, respectively. Calibration curves were prepared by the standard addition method. The limit of detection of manganese in serum and urine was 0.2 μg L-1 for the method with deuterium correction and 0.1 μg L-1 for the method with Zeeman correction. The values for manganese in sera of 142 occupationally exposed workers ranged between 0.80 and 7.20 μg L-1 (mean ± SD 2.16 ± 1.18 μg L-1) while for 31 nonexposed subjects the value ranged from 0.75 to 2.30 μg L-1 (mean ± SD 1.73 ± 0.77 μg L-1).