© 2020 Elsevier B.V.In this work, poly(ethylene glycol dimethacrylate-N-methacryloyl-L-tryptophan methyl ester) [poly(EGDMA-MATrp)] microbeads (average diameter = 106–300 µm), were synthesized by suspension polymerization technique. The microbeads showed strong affinity and allowed for solid phase extraction (SPE) of two common pesticides; pirimicarb and propoxur in environmental water samples. Some SPE process parameters such as the desorption solvent type, solvent volume, pH and salt concentration were optimized. When it was coupled with high pressure liquid chromatography (HPLC-PDA) for the determination of analytes, the linear ranges were about 10–800 ng mL−1 and the detection limits were 0.95–2.85 ng mL−1. Also the correlation coefficients (0.9949–0.9991) and precisions (2.15–2.52% RSD, n = 3) were determined. The technique was also applied to the enrichment of these pesticides in various environmental water samples. However, no target analyte was detected in the samples. Therefore, samples were spiked and recovery values were obtained in the range of 95.8–104.2% with RSDs of 2.6–6.6%.