Akademik Veri Yönetim Sistemi
Cumhuriyet Tıp Dergisi (ELEKTRONİK), cilt.42, sa.1, ss.79-85, 2020 (Scopus)
Objective: Hydrogen peroxide is a bleaching agent accepted as safe for
environment and used for producing cotton fabrics. This process requires
alkaline medium, stabilizer and high temperature. For this purpose, it is
used extensively in the textile industry. Potassium permanganate is a widely
used agent in our country as it is easier to obtain compared to other oxidizing
agents. In this study, we investigated whether occupational exposure to
potassium permanganate causes genotoxic effect or not.
Method: This cross-sectional study was carried out in a denim factory
located in the Organized Industrial Zone in Sivas. The total number of
employees was 600 in the factory including 50 workers in the office and 550
workers in the production department.
The study group was consisted of 32 healthy males, non-smokers, without
chronic diseases and infections. They had been working at least 2 years in
denim bleaching unit. The control group was consisted of 30 healthy male
volunteers, at similar age, non-smokers, working in administrative or desk
jobs in the same factory, with little or no risk of chemical exposure. Workers
who had an infection in the last month and who were on medication were
excluded from the study. In Vitro Micronucleus Test was used to determine
the genotoxic effect of potassium permanganate. The venous blood samples
taken from the workers and controls was cultured in laboratory conditions
according to Fenech’s method with minor modifications. Evaluation of
slides was carried out according to Fenech’s microscopic survey criteria at
×1000 magnification. The frequencies of the total number of micronuclei in
the lymphocytes (MNL) and bi-nucleated cells with the micronuclei
(BNMN) were determined. In addition, to determine the frequencies of
nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs), 1000 nucleated
cells with well-preserved cytoplasm were also evaluated. Student’s T-test
was used for statistical evaluation.
Results: When groups were compared, the micronucleus frequency was
detected as about twice high in study group. Statistically significant
difference was detected between the study and control group (p<0.05). The
nuclear bud frequency was significantly higher in study groups than in
controls (p<0.05). Although the number of nucleoplasmic bridges were
higher in study group, this difference was not statistically significant
(p>0.05).