Akademik Veri Yönetim Sistemi
2023 Annual Congress of the Swiss Society for Allergology and Immunology (SGAI-SSAI), Bern, İsviçre, 24 - 25 Ağustos 2023, sa.39, ss.18
Aim: We investigated the effects of food emulsifiers and professional dishwasher detergents on cytotoxicity, barrier function, transcriptome alterations, and protein expression in gastrointestinal epithelial cells.
Design & Methods: Human intestinal organoids, organoid organ-on-a-chips, and liquid-liquid interface cells were cultured in the presence of two emulsifiers, polysorbate 20 (P20) and polysorbate 80 (P80), and dishwasher detergent and rinse aid.
Results: Cells showed lysis in response to P20 and P80 exposure starting at a 0.1% concentration and to rinse aid at 1:20,000 dilution. Epithelial barrier disruption correlated with decreased TEER, increased paracellular-flux. Alcohol ethoxylated found in rinse aid were identified as the culprit with a strong cell toxic and epithelial barrier damaging effect. RNAseq and targeted-proteomics analyses demonstrated upregulation of pathways related to cell development, proliferation, apoptosis, inflammatory response and response to stress at 0.05% concentration. In addition, immune and inflammatory responses, cell death, migration, epithelial development, proliferation, were significantly upregulated in response to rinse aid. Epithelial cells incubated with residue from professional dishwashers demonstrated the presence of a significant amount of cytotoxic detergents remaining on ready to use dishware.
Conclusions: Our studies provide evidence on the detrimental effects of food emulsifiers and rinse aid on gut epithelial cell integrity. Even at concentrations lower than the toxic doses, they induced proinflammatory responses.