Epitopic characterisation of Turkish bovine viral diarrhoea virus (BVDV) isolates using monoclonal antibodies

Yesilbag K., Burgu I.

ACTA VETERINARIA HUNGARICA, cilt.51, sa.2, ss.237-244, 2003 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 2
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1556/avet.51.2003.2.12
  • Dergi Adı: ACTA VETERINARIA HUNGARICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.237-244
  • Anahtar Kelimeler: BVDV, cloning, antigenic characterisation, monoclonal antibodies, MUCOSAL DISEASE, STRAINS, IDENTIFICATION, BIOTYPES, CATTLE, ASSAY
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

In this study, 15 bovine viral diarrhoea viruses (BVDV) isolated from the field in Turkey were characterised for their biotype, cloned and eventually analysed for their epitopic composition in terms of glycoprotein E2. Immunoplaque assay, plaque assay, limiting dilution and streptavidin-biotin-peroxidase techniques were used for biotype characterisation, cloning of cytopathic (cp) and noncytopathic (ncp) biotypes and epitope analysis, respectively. While 14 out of 15 BVDV isolates were distinguished as ncp biotype, I isolate was found to be containing both biotypes (cp + ncp). According to the reactivity patterns of isolates with 15 monoclonal antibodies, 4 different antigenic groups could be formed. There were no antigenic differences between the isolates derived from the same animal with various time intervals. On the other hand, biotype clones isolated from the same animal exhibited difference in one epitope. This is the first study describing antigenic characterisation of BVDV field isolates in Turkey.