Here, Pseudomonas spp. molecularly imprinted polymer-based surface plasmon resonance (MIP-based SPR) sensor has been prepared to perform quantitative analysis of Pseudomonas spp simultaneously. The polymeric nanofilm is imprinted on the SPR sensor surface with the microcontact printing method. Nanofilm was characterized with contact angle Non-Imprinted Polymer (NIP) and MIP SPR chips were found at 68.7 degrees and 79.5 degrees, respectively), ellipsometer measurement (the thickness of MIP and NIP nanofilm determined 150.4 +/- 1.5 nm and 72.4 +/- 1.2 nm values, respectively) and SEM analysis. The different Pseudomonas spp. were prepared at a concentration range of 1 x 10(2) - 1 x 10(4) CFU/mL and passed through the SPR system. The total detection time for all steps was reported in 150 seconds for microorganism detection, which is very short compared to the cultural method (2 days). The detection of limit value was calculated 0.5 x 10(2) CFU/mL. Selectivity of Pseudomonas spp. nanofilm based SPR sensors %Delta R value was determined 8.3 for MIP and 0.25 for NIP. The relative selectivity coefficients (k') of MIP nanofilm based SPR sensor for Staphylococcus aureus, Salmonella paratyphi and Escherichia coli were reported 25.82, 31.69 and 31.24 times, respectively. According to the data obtained in this study, the prepared Pseudomonas spp, suppressed surface plasmon resonance sensor, can be used as an alternative to the current cultural method and effective suppression quality, selectivity, and affinity.