AN INVESTIGATION OF THE ACE INHIBITORY ACTIVITY, ANTIOXIDANT CAPACITY, AND PHYTOCHEMICAL CONSTITUENTS OF POLAR AND NON-POLAR EXTRACTS OF ZIZIPHUS JUJUBA FRUIT: STATISTICAL SCREENING THE MAIN COMPONENTS RESPONSIBLE FOR BIOACTIVITY

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FINDIK B. T., Yildiz H., Birisci E., Yiğitkan S., Koseoglu Yilmaz P., Ertaş A.

GIDA, vol.49, no.3, pp.554-566, 2024 (Peer-Reviewed Journal) identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 3
  • Publication Date: 2024
  • Doi Number: 10.15237/gida.gd24028
  • Journal Name: GIDA
  • Journal Indexes: TR DİZİN (ULAKBİM)
  • Page Numbers: pp.554-566
  • Bursa Uludag University Affiliated: Yes

Abstract

Herein, the angiotensin I-converting enzyme (ACE) inhibitory activity, antioxidant capacity, total polyphenol contents (TPC), and phytochemical profiles of polar and non-polar extracts of dried Ziziphus jujuba fruits were investigated, along with the statistical determination of the main components responsible for ACE inhibitory activity. The non-polar extract expressed the strongest ACE inhibitory activity (99.81%) among the extracts. The non-polar extract also exhibited the highest DPPH scavenging activity (IC50 of 30.63), linoleic acid/β-carotene bleaching capacity (89.31%), and TPC (59.47 mg GAE/g). The phenolic profiles of the extracts were identified by LC-MS/MS, and the presence of seven triterpenoid species in the extracts was examined using GC-MS techniques. The principal constituents included 19 phenolics, 2 organic acids, and 4 triterpenoids. A Pearson correlation and principal component analysis were conducted to find the correlation between individual phenolic compounds and ACE inhibitory activity.