Akademik Veri Yönetim Sistemi
IBRO 2023, Granada, İspanya, 9 - 13 Eylül 2023, ss.144
Valproic acid (VPA) is an environmental risk factor of Autism Spectrum Disorders (ASD), especially during first trimester, that is widely used in experimental designs for its ability to model ASD both morphologically and behaviourally on animals but not on cell lines. Cell lines provide cheaper, ethically less problematic and reusable models. Aim of this study was to use SH-SY5Y human neuroblastoma cell line to develop an in vitro model for ASD and observing VPA’s effects directly on cells during proliferation. SH-SY5Y cells were seeded to 96-well plate with 2500 cells per well. 24hrs after seeding the media was changed and VPA (dissolved in DMEM/F12) was introduced to wells. Experimental groups were as followed; A: Control group (vehicle), B: Groups that were exposed to different doses of VPA (B-1mM, B-5mM, B10mM). VPA exposures were for 24hrs. The MTT assay and cell counting by trypan blue were performed as triplicated to determine cell viability. Statistical analysis was performed on Sigma Plot. MTT assay revealed that B-10mM had significantly less cells compared to A, B-1mM and B-5mM (p<0,05; p<0,05; p<0,05), whereas B-5mM had less cells compared to A and B-1mM (p<0,05; p<0,05). There was no significant difference between groups A and B-1mM. On the other hand, cell counting by trypan blue showed statistically less cells only on group B-10mM compared to A, B-1mM and B-5mM (p<0,05; p<0,001; p<0,01). There was not any significant difference between A and B-5mM. B-1mM had significantly more cells compared to A (p<0,05). Consistent with literature, our results showed that 1mM of VPA does not have negative effects on cell viability whereas 10mM showed detrimental effects. We suggest that 5mM VPA should be used for further analysis for cellular and morphological parameters that are disrupted in in vivo models of ASD.