Detection of IgG antibody to Bovine Leukaemia Virus in urine and serum by two enzyme immunoassays

Carli K. T., Sen A., Batmaz H., Kennerman E.

LETTERS IN APPLIED MICROBIOLOGY, vol.28, no.6, pp.416-418, 1999 (SCI-Expanded) identifier identifier identifier


Four hundred blood sera from a cattle production unit were tested for BLV-(Bovine Leukaemia Virus) antibody with IP (Institut Porquier) and SE (Svanova Biotech) ELISA kits. Seventy-seven cattle with BLV-antibody (19.25%) and 77 without the antibody were used. No significant difference was found between O.D. of sera of PL + (Persistent Lymphocytosis Positive) and PL-(Negative) cattle. The mean O.D. of urine samples of 77 seropositive cattle was significantly higher than that of 77 seronegative cattle (P < 0.01). There were also differences between urine O.D.s of seropositive (PL +) and seropositive (PL-) groups of cattle with IP (P < 0.05) and SB (P < 0.01) kits. All the results revealed the presence of BLV-antibody in the urine of the cattle without any urinary dysfunction.