Assessment of toxicity and oxidative DNA damage of sodium hypochlorite, chitosan and propolis on fibroblast cells

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Aydin Z. U., Akpinar K. E., Hepokur C., Erdönmez D.

Brazilian Oral Research, vol.32, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32
  • Publication Date: 2018
  • Doi Number: 10.1590/1807-3107bor-2018.vol32.0119
  • Journal Name: Brazilian Oral Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Chitosan, Propolis, Sodium hypochlorite
  • Bursa Uludag University Affiliated: No


© 2018. Brazilian Oral Research. All Rights Reserved.The objective of this study was to evaluate and compare thecytotoxicity and genotoxicity on human fibroblast cell lines of sodiumhypochlorite (NaOCl), chitosan and propolis as root canal irrigatingsolutions. Human fibroblast cells were exposed to chitosan, propolisand NaOCl for 4 and 24 h. Cell viability was assessed by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide,and oxidative DNA damage was assessed by determination of8-hydroxydeoxyguanosine (8-OHdG) level with an ELISA kit. The dataof cell cytotoxicity were analysed statistically using a test of one-wayanalysis of variance at a significance level of p < 0.05. In the NaOCIgroup, the 8-OHdG level was higher than in the chitosan group, butthere was no statistical difference when compared with the othergroups (p < 0.05). It was determined that the irrigation solutions werecytotoxic, depending on the dose and time. NaOCl was the most toxicsolution after both 4and 24 h of exposure (p < 0.05). Chitosan andpropolis may be alternatives to NaOCl for irrigation solutions, becausethey are both less toxic and produce less oxidative DNA damage