Solid lipid nanoparticles: Reversal of tamoxifen resistance in breast cancer
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.120, ss.73-88, 2018 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 120
- Basım Tarihi: 2018
- Doi Numarası: 10.1016/j.ejps.2018.04.040
- Dergi Adı: EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.73-88
- Anahtar Kelimeler: Breast cancer, Tamoxifen, Drug resistance, Solid lipid nanoparticles, Apoptosis, CONTROLLED DRUG-DELIVERY, CELLS, APOPTOSIS, MICRORNA, THERAPY, MIRNAS, MCF-7, SLN
- Bursa Uludağ Üniversitesi Adresli: Evet
Özet
The objective of the present study was to investigate the effect of tamoxifen (Tam) loaded solid lipid nano-particles (SLNs) on MCF7 Tam-resistant breast cancer cells (MCF7-TamR). Tam-SLNs were produced by the hot homogenization method. The characterization studies of Tam-SLNs demonstrated good physical stability with small particle size. The in vitro cytotoxicity results showed that Tam-SLNs enhanced the efficacy of Tam and reversed the acquired Tam resistance by inducing apoptosis, altering the expression levels of specific miRNA and the related apoptosis-associated target-genes in both MCF7 and MCF7-TamR cells without damaging the MCF10A control cells (p < 0.05). In conclusion, we demonstrated a molecular mechanism of the induction of apoptosis by Tam-SLNs in MCF7 and MCF7-TamR cells, and thus, we demonstrated that Tam-SLNs were more effective than Tam. The present study suggests that the use SLNs may be a potential therapeutic strategy to overcome Tam-resistance in breast cancer for clinical use.