Solid lipid nanoparticles: Reversal of tamoxifen resistance in breast cancer

Eskiler G. G., ÇEÇENER G., DİKMEN G., EGELİ Ü., TUNCA B.

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, vol.120, pp.73-88, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 120
  • Publication Date: 2018
  • Doi Number: 10.1016/j.ejps.2018.04.040
  • Journal Name: EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.73-88
  • Keywords: Breast cancer, Tamoxifen, Drug resistance, Solid lipid nanoparticles, Apoptosis, CONTROLLED DRUG-DELIVERY, CELLS, APOPTOSIS, MICRORNA, THERAPY, MIRNAS, MCF-7, SLN
  • Bursa Uludag University Affiliated: Yes

Abstract

The objective of the present study was to investigate the effect of tamoxifen (Tam) loaded solid lipid nano-particles (SLNs) on MCF7 Tam-resistant breast cancer cells (MCF7-TamR). Tam-SLNs were produced by the hot homogenization method. The characterization studies of Tam-SLNs demonstrated good physical stability with small particle size. The in vitro cytotoxicity results showed that Tam-SLNs enhanced the efficacy of Tam and reversed the acquired Tam resistance by inducing apoptosis, altering the expression levels of specific miRNA and the related apoptosis-associated target-genes in both MCF7 and MCF7-TamR cells without damaging the MCF10A control cells (p < 0.05). In conclusion, we demonstrated a molecular mechanism of the induction of apoptosis by Tam-SLNs in MCF7 and MCF7-TamR cells, and thus, we demonstrated that Tam-SLNs were more effective than Tam. The present study suggests that the use SLNs may be a potential therapeutic strategy to overcome Tam-resistance in breast cancer for clinical use.