THROMBOELASTOGRAPHIC EVALUATION OF HEMOSTATIC FUNCTION IN DOGS NATURALLY INFECTED WITH CANINE DISTEMPER VIRUS


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Kocatürk M.

9TH INTERNATIONAL EUROASIA Congress on Scientifıc Researches and Recent Trends 9, Antalya, Turkey, 19 - 20 February 2022, vol.9, pp.395

  • Publication Type: Conference Paper / Summary Text
  • Volume: 9
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.395

Abstract

Thromboelastography (TEG) has been accepted as a sensitive and useful test compared with traditional tests (prothrombin time-PT and activated partial thromboplastin time-aPTT) for evaluating coagulation status in dogs. This study aimed to evaluate hemostatic function by TEG and determine changes in hemostatic parameters in dogs with Canine Distemper virus (CDV). Eleven client-owned dogs and 10 healthy-control dogs (body-weight: 8±1.6 kg; age: 3-4.5 months) presented to Veterinary Teaching Hospital, Bursa Uludag University, Bursa/Turkey were evaluated. CDV was diagnosed by clinical and haematological findings in combination with positive faecal and blood diagnostic tests (Antigen Rapid CDV Test, Animal Genetics, Inc., Suwon/Korea). Exclusion criterias were co-infecion with other viral (parvovirus and/or coronavirus), parasitic (coccidiosis, giardiasis, ascaridiosis etc.), or vector-borne diseases by faecal screening tests (CPV+CCV+Giardia Triple Ag-snap ELISA, Biotech/China), fecal microscopic examinations, and speed ehrlichiosis, anaplasmosis, lyme, and dirofilariasis tests (Anigen Rapid CaniV-4, Bionote/Korea), respectively. Hemogram (VH5®Fuji-Japan) and serum biochemistry (Comprehensive Profile, Fuji/Japan) were evaluated. Hemostatic functions were assessed by clotting times (aPTT and PT; VetScan VSpro, Abaxis) and TEG (TEG 5000, Haemonetics, Braintree, MA/USA). TEG peremeters included were reaction time (R), coagulation time (K), alpha (α) angle, maximum amplitude (MA), G value, LY30 (percentage of lysis 30 min after MA), and coagulation index (CI). TEG of dogs with CDV revealed significantly higher R (3.2±0,4 min) and CI values (3.7±0.5) than controls (1.5±0.3 min; 1.5±0,8, P<0.05, respectively). MA (70.1±1.7 min.), α-angle (72.4±1.8 degrees), and G (12.2±1.0 dyn/cm2) were lower (P<0.05) in dogs with CDV compared to controls (63±4.1 min, 69.6±3.2 degrees, 9.2±1.5 dyn/cm2), respectively. LY30 did not statistically differ between groups. TEG profiles showed hypercoagulation, secondary fibrinolysis, or platelet dysfunction (hypocoagulation) in some cases of CDV. Study indicate that individual results were compatible with hypercoagulation state, however, coagulation is a dynamic process and can change to hypercogaulation in dogs with CDV.