Autologous Platelet Rich Plasma Have Positive Effect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season

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ALÇAY S., Aktar A., KOCA D., Kilic M. A., Akkasoglu M., Yilmaz M. M., ...Daha Fazla

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.28, ss.229-234, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28
  • Basım Tarihi: 2022
  • Doi Numarası: 10.9775/kvfd.2021.26763
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.229-234
  • Anahtar Kelimeler: Ram semen, Platelet-rich plasma, Cryopreservation, Post-thawed, EGG-YOLK, INCUBATION RESILIENCE, SOYBEAN LECITHIN, OXIDATIVE STRESS, SPERM, QUALITY, EXTENDERS, FROZEN, ANTIOXIDANTS, FERTILITY
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The aim of this study was to evaluate the effects of autologous platelet-rich plasma (PRP) supplemented egg yolk based extender on ram semen after thawing. Semen samples were collected from nine Kivircik rams and each semen was divided into four equal aliquots and mixed with different concentrations of PRP supported extenders [no PRP (control), 12.5x10(6)/mL PRP, 25x10(6)/mL PRP, or 50x10(6)/mL PRP)]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed after thawing. The results showed that 25x10(6)/mL PRP group had positive effect on motility (61.67 +/- 3.81), membrane functional integrity (71.00 +/- 2.96) and MDA levels (5.13 +/- 0.64) at post-thawed (P<0.05). It was determined that 25x10(6)/mL PRP and 50x10(6)/mL PRP groups were more effective than other groups in terms of mitochondrial membrane potential (69.50 +/- 1.93), acrosome integrity (78.04 +/- 2.65) and DNA integrity (5.33 +/- 0.92). The results of the study showed that autologous PRP has a protective effect in the cryopreservation of ram spermatozoa.