DEVELOPMENT AND VALIDATION OF A FAST MULTI RESIDUE METHOD FOR 15 HIGH POLAR PESTICIDES IN TOMATO SAMPLES USING LC-MS/MS

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Hazarhun G., Gümül B., Maden B., Arabacı E. E., Ertaş S., Kumral N. A.

2. Gıda Kimyası Kongresi, Antalya, Türkiye, 15 - 19 Mart 2023, ss.7

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.7
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The high polar chemicals, such as aminomethylphosphonic acid, bromate, bromide, chlorate, chlormequat, cyromazine, diquat, ethephon, fosetly-al, mepiquat, N-acetyl-glufosinate, perchlorate, phosphonic acid, propylene-thiourea and trimethyl sulfonium, are plant growth regulators, fertilizers, pesticides or pesticide metabolites. Their residual level in fresh foods is regulated in European Union and Türkiye. But, direct analysis of these compounds have always been challenging since they cannot be extracted easily using Quick Polar Pesticides Extraction (QuPPe) method1. Unstable retention times and chromatographically very long detection periods are the other major problems of the analysis2. It is necessary to develop a more rapid and robust chromatographic analysis method. In this study, a multi-residue analysis method was developed and verified for the detection of polar compounds in tomato matrix using LC MS/MS. The compounds were extracted using a methanol-based very short sample preparation method. All 15 compounds were analysed simultaneously in a single run with a total analysis period of less than 4 minutes though changing column specification and buffer formulation. The validation data of all compounds obtained in LC-MS/MS analysis was quite reliable and were in line with the SANTE 11312/2021 Guideline3. Calibration results for 6 concentrations from 10 to 400 μg kg−1, showed good linearity (R2 = 0.990 – 0.999). In addition, interference and retention time shift were not detected. Average recovery rates were between 84.03 and 119.65% for all compounds, when tested with 10 and 50 µg kg-1 concentrations, respectively. The maximum RSD values of repeatability (RSDr) varied between 0.74 and 18.31% for same concentrations of the compounds. The maximum RSD values of reproducibility (RSDwR) for same concentrations were between 2.94 and 18.62%. Low LOQ values (6.00 to 8.09 μg kg−1) were achieved for all compounds. The validated LOQ values were under the lowest Maximum Residue Limits of European Union of the compounds for tomato sample. This study was funded by Perla Fruit Company with the grant number ULUTEK STB079417.