In this study cattle, sheep and goat populations in an isolated island eco-system (Gokceada, Imbros) were serologically investigated against viral infections which are important for animal health an herd management in these animal species. In this content, 75 cattle, 161 sheep and 30 goat serum samples are screened for antibodies against bovine viral diarrhoea virus (BVDV), bovine herpesvirus-1 (BoHV-1), bovine respiratory syncytial virus (BRSV), parainfluenza virus type-3 (PI-3), bovine adenovirus-1 (BAV-1), bovine adenovirus-3 (BAV-3), and bluetongue virus-4 (BTV-4) by using serum neutralization assay. Moreover bovine leukemia virus (BLV) antibodies were screened in cattle sera by ELISA. According to the test results, seroprevalance of mentioned infections among the animal species were 14.6%, 58.6%, 26.6%, 29.3%, 72.0%, 68.0%, and 46.6% in cattle, 1.8%, 0.0%, 50.9%, 11.8%, 91.3%, 75.1%, and 5.6% in sheep, 0.0%, 26.6%, 70.0%, 3.3%, 83.3%, 66.6%, and 6.6% in goats, respectively. Seroprevalance rate in 266 tested animals were determined as 5.2%, 24.3%, 46.2%, 15.7%, 84.9%, 72.1%, and 17.2% respectively. There were no cattle that produced positive result for BLV antibodies. Compared to the other viruses, BAV-1 and BAV-3 represented the highest seroprevalance rates in all the species. Detecting no animal that is positive for BLV antibodies may indicate the status of the investigated ecosystem as to be free from this infection.