Akademik Veri Yönetim Sistemi
13th International Medicine and Heath Sciences Researches Congress, Ankara, Türkiye, 26 - 27 Ağustos 2023, sa.437, ss.582-583
Aim: There is a tight relationship established between the neuroendocrine regulation of
metabolism and reproduction. Nesfatin-1 is a neuropeptide that suppresses food intake, while kisspeptin is involved in the regulation of reproduction. The expression of Nesfatin-1 neurons has been shown in
the hypothalamic ARC, PVN, SON, and lateral hypothalamic area. The expression of kisspeptin
neurons, has been demonstrated in the ARC, APN, and PeV. Although there are studies showing similar
mechanisms for both peptides, a study demonstrating the possible interactions between these two
peptidergic systems on each other has not been encountered in the literature. In this study, it is aimed to
demonstrate possible presynaptic terminations between nesfatin-1 and kisspeptin neurons using
immunohistochemical techniques. Methods: Male (n=5) and female (n=5) Sprague Dawley rats
weighing 200-300 g were used. Coronal sections with a thickness of 50 μm were obtained through
vibratome from control subjects that were sacrificed through perfusion fixation. Dual
immunoperoxidase immunohistochemistry with nesfatin-1 and kisspeptin antibodies was performed on
sections obtained.Nesfatin-1 neurons were evaluated under light microscopy in terms of their contacts
with axon terminals containing kisspeptin. In accordance with the literature, nesfatin-1 neurons that had
at least one contact with a kisspeptin-positive axon terminal were considered as 'innervated neurons,'
and the ratio of these neurons to all nesfatin-1 neurons was calculated. Results: When comparing
kisspeptin-positive presynaptic terminations on nesfatin-1 neurons localized in the PVN, it was
determined that male subjects received innervation at a rate of 93.03±1.16%, whereas female subjects
received innervation at a rate of 73.77±5.13%. The statistical analysis has revealed that the difference
between the two groups is statistically significant (p=0.006). Conclusion: As a result of our study, it has
been established that nesfatin-1 neurons localized in the PVN receive innervations from kisspeptinergic
neuron terminals, and gender-related variations are evident between male and female subjects.
Keywords: Nesfatin-1, Kisspeptin, Food Intake, Immunohistochemistry, Rat.