Dietary intake of Spirulina platensis alters HSP70 gene expression profiles in the brain of rats in an experimental model of mixed stress

Ardıclı S., Seyıdoglu N., Koselı E., Gurbanlı R., Aydın C.

JOURNAL OF GENETICS, cilt.2022, sa.101, ss.1-11, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 2022 Sayı: 101
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s12041-022-01388-5
  • Dergi Adı: JOURNAL OF GENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1-11
  • Anahtar Kelimeler: gene expression, HSP70 gene, real-time polymerase chain reaction, Spirulina (Arthrospira) platensis, stress, HEAT-SHOCK FACTOR, C-PHYCOCYANIN, OXIDATIVE STRESS, MESSENGER-RNA, SUPPLEMENTATION, LIPOPOLYSACCHARIDE, INFLAMMATION, ANTIOXIDANT, RESISTANCE, IMMUNITY
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Spirulina platensis has gradually gained more attention for its therapeutic, antioxidant, and anti-inflammatory potential

worldwide. However, the current molecular knowledge about the effects of spirulina on stress-related genes is rather limited. The effects of

dietary intake of spirulina on the HSP70 gene expression were assessed in a controlled in vivo experimental design. Moreover, alterations in

serum corticosterone levels, IL-2, IL-4, IFN-c, triglyceride, ALT, AST, relative gene expression values, and the correlations between them

were evaluated. A total of 36 rats were divided into four groups: control group, stress-only group, spirulina group, and spirulina?stress

group. To control the dose administration, S. platensis was applied by a gastric gavage in stress groups. Crowded environment stress and

hosting alone stress were applied to the stress-only group and spirulina ? stress group. RNA was extracted from brain samples using

TRIpure and the relative gene expression assessment was performed using Roche-LightCycler-480-II real-time PCR-System. Gene

expression values were remarkably different among the four experimental groups. The differences between stress-only and the spirulina

groups were statistically significant (P\0.05). The correlation between the HSP70 gene expression and the IFN-c was found to be

statistically significant (P\0.05; r=0.50). Results indicate a novel effect of spirulina on the HSP70 expression related to the stress-response.

Data presented in this study may be useful for further studies to define not only the molecular genetic aspects through dietary S. platensis

but also the effects of spirulina on stress-response and animal welfare.