Military staff are required to have a high level of fitness to be able to cope with the physical challenges of his profession to perform the tasks assigned to him and to be protected from the possible injuries. Therefore, their strength and cardiovascular endurance parameters are measured and evaluated by tests. The aim of present study was to determine the relationship between the multistage 20 m shuttle run test(MSRT) and 1200 m, 1600 m, 2400 m running tests performed on the military high school students and the number of shuttles that counterbalance different running times. In present study, 588 volunteer military school students were recruited in this study and categorized in 3 groups according to age. In the first group (G1) 15 ages (n = 168, height 171 +/- 6,22 cm, weight 64 +/- 8.69 kg), in the second group (G2) 16 ages (n = 121, height 175 +/- 5.14 cm, weight 65 +/- 7.21 kg) ad the third group (G3) 17 and 18 ages (n = 300, height 177 +/- 5.18 cm, weight 70.5 +/- 7.55 kg) took place. G1, G2 and G3 groups performed 1200 m, 1600 m, 2400 m running tests, respectively the running speed were set. All of the students performed the shuttle run test two days later. The relationship between the number of shuttles and running speed were analyzed by multiple regression analysis. It determined following formulas. Formula 1: 1200 m n of shuttles for running = (6.893 x 1200 m running speed (km.h(-1))) + (-22.513), Formula 2: 1600 m n of shuttles for running = (8.889 x 1600 m running speed (km.h(-1))) + (-43.548), Formula 3: 2400 m n of shuttles for running = (7.691 x 2400 m running speed (km.h(-1))) + (18.410). In conclusion, there are positive relationship between the shuttle run test and 1200 m, 1600 m, 2400 m running tests. Also, we can say military high school students could be used the shuttle run test instead of other distance running tests to determine cardiovascular endurance.