In this paper, thermal and hydraulic analysis of a dual-separated cooling channel was performed for the cooling applications of different automotive lighting systems. A single LED and multi-LEDs were considered to evaluate the thermal and hydraulic performance as well as the lighting properties of automotive headlights. The detailed hydraulic analysis was theoretically evaluated for developing laminar flow to get higher thermal performance with less pumping power. The theoretical and simulation results for the dual-separated cooling channel were compared and discussed. Unlike the constant properties were used in the current literature, thermal analysis with temperature-dependent properties was performed to estimate Nusselt number preciously for dual-separated cooling channels. The temperature measurements were also performed in the experimental study to compare the numerical results. The light output parameter of the LEDs depends on junction temperature, it was found that the increase in light output by using a dualseparated cooling channel was about 10% for all cases. Otherwise, higher junction temperature values lead to a reduction in operating efficiency. The top copper surface temperature drop was higher than 50%, and the junction temperature had been reduced by 36% to ensure the desired operating conditions of the automotive lighting system.