Analytical Grade Purification of Phycocyanin from Cyanobacteria.

Khazi M. I. B., Demirel Z., Liaqat F., Dalay M. C.

in: Biofuels from Algae, Kristian Spilling, Editor, Humana Press, Inc. , New York, pp.173-179, 2019

  • Publication Type: Book Chapter / Chapter Research Book
  • Publication Date: 2019
  • Publisher: Humana Press, Inc.
  • City: New York
  • Page Numbers: pp.173-179
  • Editors: Kristian Spilling, Editor
  • Bursa Uludag University Affiliated: Yes

Abstract

Phycocyanin is a blue-colored pigment-protein complex that exhibits numerous biofunctions such as antiinflammation, antioxidation, antitumor, neuroprotective effect, and immunological enhancement. Purified phycocyanin has pharmaceutical and nutraceutical applications. In addition, as a nontoxic and non-carcinogenic natural coloring agent, phycocyanin has many applications in the food and cosmetic industries. This chapter describes a protocol for extraction and analytical grade purification of phycocyanin from cyanobacteria. The purification steps include (1) extraction of phycocyanin from biomass, (2) ammonium sulfate precipitation of phycocyanin and dialysis, and (3) purification of phycocyanin by gel filtration and ion-exchange chromatography.