Analytical Grade Purification of Phycocyanin from Cyanobacteria.

Khazi M. I. B., Demirel Z., Liaqat F., Dalay M. C.

Biofuels from Algae, Kristian Spilling, Editör, Humana Press, Inc. , New York, ss.173-179, 2019

  • Yayın Türü: Kitapta Bölüm / Araştırma Kitabı
  • Basım Tarihi: 2019
  • Yayınevi: Humana Press, Inc.
  • Basıldığı Şehir: New York
  • Sayfa Sayıları: ss.173-179
  • Editörler: Kristian Spilling, Editör
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Phycocyanin is a blue-colored pigment-protein complex that exhibits numerous biofunctions such as antiinflammation, antioxidation, antitumor, neuroprotective effect, and immunological enhancement. Purified phycocyanin has pharmaceutical and nutraceutical applications. In addition, as a nontoxic and non-carcinogenic natural coloring agent, phycocyanin has many applications in the food and cosmetic industries. This chapter describes a protocol for extraction and analytical grade purification of phycocyanin from cyanobacteria. The purification steps include (1) extraction of phycocyanin from biomass, (2) ammonium sulfate precipitation of phycocyanin and dialysis, and (3) purification of phycocyanin by gel filtration and ion-exchange chromatography.