The Ala Allele at Val762Ala Polymorphism in Poly(ADP-ribose) Polymerase-1 (PARP-1) Gene is Associated with a Decreased Risk of Asthma in a Turkish Population


Tezcan G. , Gurel C. B. , Tutluoglu B., Onaran I., Kanigur-Sultuybek G.

JOURNAL OF ASTHMA, vol.46, no.4, pp.371-374, 2009 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 46 Issue: 4
  • Publication Date: 2009
  • Doi Number: 10.1080/02770900902777791
  • Title of Journal : JOURNAL OF ASTHMA
  • Page Numbers: pp.371-374
  • Keywords: asthma, genetic susceptibility, SNP, poly(ADP-ribose) polymerase-1, MURINE MODEL, INHIBITION, ACTIVATION, DEFICIENT, VARIANTS, CANCER, INFLAMMATION, MIGRATION, SHOCK

Abstract

Background and objective. It has been suggested that inhibition of poly (ADP-ribose) polymerase-1 (PARP-1), either pharmacologically or by a gene knockout provides significant protection against systemic or tissue inflammation in animal models. The aim of this study was to analyze the association of the PARP-1 Val762Ala polymorphism, which has beenreported to be associated with decreased enzymatic activity, in Turkish patients with adult asthma. Methods. A total of 112 subjects with stable asthma and 180 normal controls from the same geographic region were studied and polymerase chain reaction-based restriction analysis was used to identify Val762Ala polymorphism of the PARP-1. Results. In univariate analysis, PARP-1 762 AA genotype conferred a 3.4 fold reduction in risk (OR = 0.297, 95% CI = 0.105-0.813; P = 0.014), while heterozygous VA genotype conferred an even greater level of protection (OR = 0.06; 95%CI, 0.026-0.14; P 10-6). In addition, wild type PARP-1 762 V allele had 5 times the risk of developing asthma than those without the allele (OR 0.199, CI 0.118-0.334, P = 10-6). Conclusions. These findings suggest that PARP-1 V762A variants may be one of the factors participating in protection or susceptibility to asthma in our population.